Identification of Proteins Adsorbed on Hydroxyapatite Ceramics with a Preferred Orientation to a-Plane

Protein adsorption is essential for determining material biocompatibility and promoting adherent cell growth. In this study, we focused on the a-plane structure of hydroxyapatite (HAp). This closely resembles crystal plane where apatite exposed in long bones. We conducted protein experiments using HAp ceramics with a preferred orientation to a-planes (aHAp), employing bovine serum albumin (BSA), lysozyme, fetal (FBS) as models mimic vivo environment. Higher zeta potential contact angle values were found aHAp than fabricated from commercial powder (iHAp). Bradford-quantified revealed BSA 212 ng·mm−2 28.4 ng mm−2 iHAp. Furthermore, FBS 2.07 μg 1.28 µg Two-dimensional electrophoresis (2D-PAGE) showed higher number protein-derived major spots (37 spots) iHAp (12 spots). Mass spectrometry analysis resulting 2D-PAGE gels proteins adsorbed aHAp, including secreted frizzled-related 3 vitamin K epoxide reductase complex 1, which are involved cellular bone differentiation. Overall, these expected promote differentiation, representing characteristic property aHAp.